Probabilistic Secondary Structure of the Protein

ADAPT-NMR provides probabilistic secondary structure of the protein in PECAN format (A jpg file and a text file).

The image and the text of PECAN output is shown as annotated images below.   The following tags are used to identify the structure.

_PECAN.Extended_probability	The probability for the given residue to be in Extended (Beta Sheet) state
_PECAN.Helix_probability	The probability for the given residue to be in Alpha Helical state
_PECAN.Extended_boundary_designator	a value of 1 (one) indicates PECAN designation of the residue as a boundary residue in an Extended region
_PECAN.Helix_boundary_designator	a value of 1 (one) indicates PECAN designation of the residue as a boundary residue in a Helical region

When the probability for a given state is less than one, the remainder indicates the probability of being in "other" state.

NPDPlot tool in NMRFAM-Sparky package can be used to analyze the result interactively.
Open "npdplot.ini" file in the NPDPlot.